3,3'Diaminobenzidine (DAB) is a widely used chromogen for
immunohistochemical staining and immunoblotting. When in the
presence of peroxidase enzyme, DAB produces a brown
precipitate that is insoluble in alcohol. A DAB buffer containing
Hydrogen peroxide is required to mix with this product. The
standard working dilution is 50ul (0.9mg) of DAB solution per 1ml
of DAB buffer, although the ratio can be adjusted as desired, the
use of liquid components reduces some risks associated with
handling powders, and eliminates waste which often results from
using tablets that require a predetermined final volume. Once the
two components are combined, the reagent can be used for up
to six hours, making it ideal for automated stainers.
