DAB buffer is a reagent specifically designed for optimal signal
intensity and contrast. When in the presence of peroxidase
enzyme and DAB solution, the buffer helps create a brown
precipitate that is insoluble in alcohol. The standard working
dilution is 50ul (0.9mg) of DAB solution per 1ml of DAB buffer,
although the ratio can be adjusted as desired, the use of liquid
components reduces some risks associated with handling
powders, and eliminates waste which often results from using
tablets that require a predetermined final volume. Once the two
components are combined, the reagent can be used for up to six
hours, making it ideal for automated stainers
